Characterization of Mid1 domains for targeting and scaffolding in fission
نویسنده
چکیده
3 I-Ju Lee and Jian-Qiu Wu 4 5 Department of Molecular Genetics, Graduate Program of Molecular, Cellular, and 6 Developmental Biology, Department of Molecular and Cellular Biochemistry, The Ohio State 7 University, Columbus, OH 43210, USA 8 9 *Address correspondence to Jian-Qiu Wu: Address: Room 615, Biological Science Building, 10 The Ohio State University, Columbus, OH 43210, USA; 11 Email: [email protected]; Telephone: (614) 247-6680; Fax: (614) 292-4466 12 13 Running Title: Mid1 domains for targeting and scaffolding 14 15
منابع مشابه
Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis.
Division-site selection and contractile-ring assembly are two crucial steps in cytokinesis. In fission yeast, the anillin-like Mid1 protein specifies the division site at the cell equator by assembling cortical nodes, the precursors of the contractile ring. Thus, Mid1 is essential for linking the positional cues for the cleavage site to contractile-ring formation. However, how Mid1 domains coop...
متن کاملCytokinesis: Does Mid1 Have an Identity Crisis?
New work shows the anillin-related protein Mid1 does not position the cytokinetic ring in the fission yeast Schizosaccharomyces japonicus, unlike its role in S. pombe. Further analysis suggests the conserved function of Mid1-like anillin proteins may be in scaffolding, not positioning, the cytokinetic ring.
متن کاملThe Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1
Cdc14 phosphatases antagonize cyclin-dependent kinase-directed phosphorylation events and are involved in several facets of cell cycle control. We investigate the role of the fission yeast Cdc14 homologue Clp1/Flp1 in cytokinesis. We find that Clp1/Flp1 is tethered at the contractile ring (CR) through its association with anillin-related Mid1. Fluorescent recovery after photobleaching analyses ...
متن کاملRewiring of Cellular Division Site Selection in Evolution of Fission Yeasts
Strategies to position the division apparatus exhibit a bewildering diversity [1], but how these mechanisms evolve remains virtually unknown. Here, we explore the plasticity of division site positioning in fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus. We demonstrate that, whereas both species divide in the middle, only S. pombe uses the anillin Mid1 as a primary nu...
متن کاملMyosin-II reorganization during mitosis is controlled temporally by its dephosphorylation and spatially by Mid1 in fission yeast
Cytokinesis in many eukaryotes requires an actomyosin contractile ring. Here, we show that in fission yeast the myosin-II heavy chain Myo2 initially accumulates at the division site via its COOH-terminal 134 amino acids independently of F-actin. The COOH-terminal region can access to the division site at early G2, whereas intact Myo2 does so at early mitosis. Ser1444 in the Myo2 COOH-terminal r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2012